Composite

Part:BBa_K3285669:Design

Designed by: PRANAV S R   Group: iGEM19_IISER-Pune-India   (2019-10-16)


Lead biosensor with improved transcription efficiency for better GFP readout.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 465
    Illegal NheI site found at 488
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 655


Design Notes

We noticed that sfGFP used in this part is incompletely sequenced due to errors in the sequence. Hence, for further usage of this, we would recommend replacing the sfGFP with GFP.


Source

The new RBS introduced into the existing biosensor biobrick was obtained from the 2019 iGEM distribution kit plates.

References